Sustainable valorisation of organic urban wastes : insights from African case studies

Understanding the problems and potentials of the organic waste stream is perhaps the single most important step that city authorities in Africa could take in moving towards sustainable, affordable, effective and efficient waste management. This publication presents four examples of recent attempts to manage organic waste sustainably in the African context. The participants in the ‘Nairobi organic urban waste’ project have structured this case exercise in order to use the case studies as object lessons, to harvest genuine insights into the feasibility of a variety of ways to successfully and sustainably valorise urban organic waste streams. Three contemporary case examples of compost production are presented. These include composting by a community-based organisation in the Kenyan private sector and by a public-private partnership in Malawi. In all three cases, the project and case study focus is on the relations between city waste and the agricultural supply chain. A fourth case study describes the technical and economic potential to produce and use biogas from urban organic waste

Human genetics and genomics research in Ecuador: historical survey, current state, and future directions

Background: In South America, the history of human genetics is extensive and its beginnings go back to the onset of the twentieth century. In Ecuador, the historical record of human genetics and genomics research is limited. In this context, our work analyzes the current status and historical panorama of these fields, based on bibliographic searches in Scopus, Google Scholar, PubMed, and Web of Science. Results: Our results determined that the oldest paper in human genetics coauthored by an Ecuadorian institution originates from the Central University of Ecuador in 1978. From a historical standpoint, the number of articles has increased since the 1990s. This growth has intensified and it is reflected in 137 manuscripts recorded from 2010 to 2019. Areas such as human population genetics, phylogeography, and forensic sciences are the core of genetics and genomics-associated research in Ecuador. Important advances have been made in the understanding of the bases of cancer, some genetic diseases, and congenital disorders. Fields such as pharmacogenetics and pharmacogenomics have begun to be explored during the last years. Conclusions: This work paints a comprehensive picture and provides additional insights into the future panorama of human genetic and genomic research in Ecuador as an example of an emerging, resource-limited country with interesting phylogeographic characteristics and public health implications

Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor

Controlled expansion and differentiation of mesenchymal stem cells in a microcarrier based stirred bioreactor

Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126

In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. LB126 were investigated. The ? and ? subunits of a dioxygenase complex (FlnA1A2), showing 63% and 51% sequence identity respectively, with the subunits of an angular dioxygenase from Gram-positive Terrabacter sp. DBF63, were identified. When overexpressed in E. coli, FlnA1A2 was responsible for the angular oxidation of fluorene, fluorenol, fluorenone, dibenzofuran and dibenzo-p-dioxin. Moreover, FlnA1A2 was able to oxidize polycyclic aromatic hydrocarbons and heteroaromatics, some of which were not oxidized by the dioxygenase from Terrabacter sp. DBF63. Quantification of resulting oxidation products showed that fluorene and phenanthrene were preferred substrates

The microbiome of the ice-capped Cayambe Volcanic Complex in Ecuador

A major challenge in microbial ecology is to understand the principles and processes by which microbes associate and interact in community assemblages. Microbial communities in mountain glaciers are unique as first colonizers and nutrient enrichment drivers for downstream ecosystems. However, mountain glaciers have been distinctively sensitive to climate perturbations and have suffered a severe retreat over the past 40  years, compelling us to understand glacier ecosystems before their disappearance. This is the first study in an Andean glacier in Ecuador offering insights into the relationship of physicochemical variables and altitude on the diversity and structure of bacterial communities. Our study covered extreme Andean altitudes at the Cayambe Volcanic Complex, from 4,783 to 5,583 masl. Glacier soil and ice samples were used as the source for 16S rRNA gene amplicon libraries. We found (1) effects of altitude on diversity and community structure, (2) the presence of few significantly correlated nutrients to community structure, (3) sharp differences between glacier soil and glacier ice in diversity and community structure, where, as quantified by the Shannon γ-diversity distribution, the meta-community in glacier soil showed more diversity than in glacier ice; this pattern was related to the higher variability of the physicochemical distribution of variables in the former substrate, and (4) significantly abundant genera associated with either high or low altitudes that could serve as biomarkers for studies on climate change. Our results provide the first assessment of these unexplored communities, before their potential disappearance due to glacier retreat and climate change

Metabolic design of macroscopic bioreaction models: application to Chinese hamster ovary cells

The aim of this paper is to present a systematic methodology to design macroscopic bioreaction models for cell cultures based upon metabolic networks. The cell culture is seen as a succession of phases. During each phase, a metabolic network represents the set of reactions occurring in the cell. Then, through the use of the elementary flux modes, these metabolic networks are used to derive macroscopic bioreactions linking the extracellular substrates and products. On this basis, as many separate models are obtained as there are phases. Then, a complete model is obtained by smoothly switching from model to model. This is illustrated with batch cultures of Chinese hamster ovary cells

Increasing the Astrophysical Reach of the Advanced Virgo Detector via the Application of Squeezed Vacuum States of Light

Current interferometric gravitational-wave detectors are limited by quantum noise over a wide range of their measurement bandwidth. One method to overcome the quantum limit is the injection of squeezed vacuum states of light into the interferometer's dark port. Here, we report on the successful application of this quantum technology to improve the shot noise limited sensitivity of the Advanced Virgo gravitational-wave detector. A sensitivity enhancement of up to 3.2±0.1 dB beyond the shot noise limit is achieved. This nonclassical improvement corresponds to a 5%-8% increase of the binary neutron star horizon. The squeezing injection was fully automated and over the first 5 months of the third joint LIGO-Virgo observation run O3 squeezing was applied for more than 99% of the science time. During this period several gravitational-wave candidates have been recorded

Stem Cell Expansion and Differentiation in Microcarrier-Based Bioreactors for Large Scale Production

Microcarriers have been widely used for various biotechnology applications because of their high scale-up potential, high reproducibility on regulating cellular behaviors, and their compliance with current Good Manufacturing Practices (cGMP). Recently, microcarriers have been investigated for stem cell expansion and differentiation, enabling potential scale-up of stem cell-derived products in large bioreactors. This presentation summarizes recent advances of using microcarriers for mesenchymal stem cell (MSC) and pluripotent stem cell (PSC) cultures in bioreactors. From the reported data, efficient expansion and differentiation of stem cells on microcarriers relies on their ability to modulate cell shape (round or spreading) and cell organization (i.e. aggregate size). Nonetheless, current screening of microcarriers remains empirical, and accurate understanding of how stem cells interact with microcarriers still remains unknown. Accurate characterization of biochemical and biomechanical properties of the microcarriers is required to fully exploit their potential in regulating stem cell fate decisions. Due to the evident variety of microcarriers, such detailed analyses should lead to the rational design of application-specific microcarriers, enabling reproducible effects on stem cell expansion and differentiation for large scale biological and biomedical applications in the pharmaceutical industry